Coding

Part:BBa_K1779201:Design

Designed by: Xingyang Qiu   Group: iGEM15_CHINA_CD_UESTC   (2015-09-04)


Integration of two genes, including mamW, RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1005
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed primers with linker of mamW, RFP. So between the two genes comprising a linker.


Source

mamW gene is from Magnetotactic bacteria's genomic sequence.RFP gene is obtained from the iGEM official bio bricks, which numbered BBa_E1010.

References